Pregnancy associated plasma protein-A(PAPP-A), pregnancy-specific 13-1 glycoprotein (SP1) and human placental lactogen(HPL), which are specific protein, have been known to be detectable in the plasma of pregnant women, especially in mid and last trimester by radioimmunoassay.
Various researches have been reported on these and the increments of them could be of value as the tumor markers and as the pregnancy related abnormalities.
Surgical specimens of normal and pathologic female genital tissues were fixed in formalin and embedded in paraffin in order to detect the exact distribution of PAPP-A, SP1 and HPL in various tissue component of them.
The result obtained are as follows :
1. Normal superficial and intermediate squamous epithelial cells, moderate and severe dysplasia, carcinoma in situ and keratinizing squamous cell carcinoma of uterine cervix revealed positive reaction to anti PAPP-A, SP1, and HPL antibodies.
2. Endometrium in both proliferative and secretory phase, endometrial hyperplasia, trophoblastic tumors revealed positive reaction to anti PAPP-A, SP1, and HPL antibody, but endometrial adenocarcinoma revealed negative reaction to each of them.
3. All of the normal ovarian tissues and various epithelial ovarian tumors revealed negative reaction to PAPP-A, SP1 and HPL antibody, but stromal and germ cell ovarian tumors revealed positive reaction to each of them.
4. Reactions to each PAPP´s were in uniformity among the same tissues, that is, once the reaction was positive, the reactions to the other remaining two PAPP´s were positive. Similarly negative reactions appeared in the same fashion.
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